Why is this Saliva Test so good?

This professional saliva drug test is a fully portable, simple to use test kit and just requires the person being tested to put the test under their tongue for ~3 mins.

Not only is this the easiest and most accurate drug test to use, it is also, as best we know, the only oral drug test that is specifically produced for the UK market and covers the 3 main street drugs found in the UK.

Principle of the Test

The Multi Saliva Drug Test (Oral Fluid) is an immunoassay based on the principle of competitive binding. Drugs that may be present in the oral fluid specimen compete against their respective drug conjugate for binding sites on their specific antibody.

During testing, a portion of the oral fluid specimen migrates upward by capillary action. A drug, if present in the oral fluid specimen below its cut-off concentration, will not saturate the binding sites of its specific antibody. The antibody will then react with the drug-protein conjugate and a visible coloured line will show up in the test line region of the specific drug strip. The presence of drug above the cut-off concentration in the oral fluid specimen will saturate all the binding sites of the antibody. Therefore, the coloured line will not form in the test line region.

A drug-positive oral fluid specimen will not generate a coloured line in the specific test line region of the strip because of drug competition, while a drug-negative oral fluid specimen will generate a line in the test line region because of the absence of drug competition.

To serve as a procedural control, a coloured line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.

Specimen Collection

1. Using the saliva collector, sweep the inside of mouth (cheek, gums, and tongue) several times, and then hold swab in mouth for at least 3 minutes, at most 7 minutes if the mouth seems to be particularly dry. Important: Do not bite, suck, or chew on the sponge.
2. Remove the saliva collector from the mouth and place into the saliva tube and press down firmly to release as much liquid as possible. Be sure that at least 0.5ml liquid has been collected so that you can re-test if necessary. If there is not enough sample then please re-insert the swab and follow from point 1 again.
3. Tightly shut the outer cap of the saliva tube and leave for 1 minute.
4. Turn the saliva tube upside down for 15 seconds to allow specimen to settle.

TEST DETECTION

Q: What drugs does the device detect and at what cut-offs?

A: A combination of Amphetamine, Cocaine, Methamphetamine, Methadone, Opiates, Marijuana and Benzodiazepines.

Q: What is the detection window compared to other drug testing methods?

A: Saliva and blood have similar detection windows. Testing saliva/blood will detect drug use faster than testing urine. Saliva/blood may detect drug ingestion immediately while drug detection in urine may take 6-8 hours post ingestion.

Q: What is a lateral flow device?

A: The oral fluids test is based on competitive binding. Drugs that may be present in the oral fluid specimen compete against their respective drug conjugate for binding sites on their specific antibody. During testing, a portion of the oral fluid sample migrates across the membrane. If no drug is present above the cut-off, the sample will not saturate the binding sites of its specific antibody. The antibody will then react with the drug-protein conjugate and a visible colored line will show up in the test line region. The presence of a drug above the cut-off concentration will saturate all the binding sites of the antibody and a line will not form in the test region. The lateral flow is the migration and competition across the membrane.

Q: Does the test quantify the concentration of drugs present in the oral fluids sample?

A: A positive test result does not indicate the concentration of drug in the sample. All positive results are presumptive and should be confirmed by an alternate method (e.g. GC/MS or GC/MS/MS). Negative results may not necessarily indicate a drug-free sample. Drug may be present in the sample below the cutoff level of the assay.

Due to the sensitive nature of these drug tests, you must be careful when carrying out the test to avoid contamination and thus inaccurate test results.

Results

You can read negative results as soon as the lines form, but for positive results please wait the full 10-minute development time. Please note the drug type code on the test case and read against the code for each drug type. Read the results of each individually and independent of one another. The top line in each result (line nearest the top of the cassette test) is the control line and is indicated by the letter “C” and the lower line (closest to base of cube case) is the test line. The control line means the test has worked correctly. If there is no upper line then the test is invalid as shown below. This means that there has been insufficient specimen volume or the instructions have not been followed correctly e.g. laying the oral cube on its side or upside down – these are the most likely reasons for the control line failing to appear.

Negative: If a second pink/red coloured line, no matter how faint, appears next to each specific drug (e.g. COC), this is a negative result.

Positive: If no lower second line appears next to any of the drugs then the individual has tested positive for that drug of abuse. The labels next to each line on the test will tell you which drug each individual strip is testing for. Remember a negative saliva sample will produce a lower line and a positive saliva sample will not produce any line below the upper control line.

Saliva Drug Test Instructions  – EN