Product Description

FOB Rapid Test Cassette detects human blood in faeces. The amount of blood increases as colon cancer, ulcers, polyps, colitis, diverticulitis, and fissures progresses.
Many diseases can cause hidden blood in the faeces. This is also known as Faecal Occult Blood (FOB), Human Occult Blood, or Human Haemoglobin. In the early stages, gastrointestinal problems such as colon cancer, ulcers, polyps, colitis, diverticulitis, and fissures may not show any visible symptoms, only occult blood. Traditional guaiac-based methods lack sensitivity and specificity, and also have diet restrictions prior to testing.

Intended Use

The FOB Rapid Test Cassette (Faeces) is a rapid chromatographic immunoassay for the qualitative detection of Human Occult Blood in faeces.

Summary

Many diseases can cause hidden blood in the faeces. This is also known as Faecal Occult Blood (FOB), Human Occult Blood, or Human Haemoglobin. In the early stages, gastrointestinal problems such as colon cancer, ulcers, polyps, colitis, diverticulitis, and fissures may not show any visible symptoms, only occult blood. Traditional guaiac based methods lack sensitivity and specificity, and also have diet restrictions prior to testing.

The FOB Rapid Test Cassette (Faeces) is a rapid test to qualitatively detect low levels of Faecal Occult Blood. The test uses a double antibody sandwich assay to selectively detect Faecal Occult Blood at 50ng/ml or higher, or 6μg/g faeces. In addition, unlike guaiac assays, the accuracy of the test is not affected by the diet of the patients.

Principle

The FOB Rapid Test Cassette (Faeces) is a qualitative, lateral flow immunoassay for the detection of Human Occult Blood in faeces. The membrane is pre-coated with anti-haemoglobin antibody on the test line region of the test. During testing, the specimen reacts with the particle coated with anti-haemoglobin antibody. The mixture migrates upward on the membrane chromatographically by capillary action to react with anti-haemoglobin antibody on the membrane and generate a coloured line. The presence of this coloured line in the test line region indicates a positive result, while its absence indicates a negative result. To serve as a procedural control, a coloured line will always
appear in the control line region, indicating that the proper volume of specimen has been added and membrane wicking has occurred.

Testing Procedure

Watch our How to Use Video to see how easy it is to check yourself!

Full instructions are included with the test which you should read through before using the test. Here is a quick illustrated guide:

What do I get in this pack?
You will receive:
1 x Specimen collection tubes with extraction buffer
1 x Test Cassettes
1 x Stool Catcher
1 x Package insert

For instructions on how to use this test click here

1. To collect faecal specimens:
   The stool specimen should be collected in the stool catcher. It is important to use the
   stool catcher to avoid contamination of the specimen with any kind of chemicals, so that no adulteration of the specimen occurs.
2. To process faecal specimens:
   Unscrew the green cap of the specimen collection tube, then randomly stab the specimen collection applicator into the faecal specimen in at least 3 different sites. Do not scoop the faecal specimen. Screw on and tighten the cap onto the specimen collection tube, then shake the specimen collection tube vigorously to mix the specimen and the extraction buffer.
   Specimens prepared in the specimen collection tube may be stored for 6 months at – 20°C if not tested within 1 hour after preparation.
3. Bring the pouch to room temperature before opening it. Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the test is performed immediately after opening the foil pouch.
4. Hold the specimen collection tube upright and remove the clear cap. Turn the tube upside down and transfer 2 full drops of the extracted specimen (approximately 80μL) to the specimen well (S) of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well (S).
5. Read results at 5 minutes. Do not read results after 10 minutes.